Isolation of Murine Skin Resident and Migratory Dendritic Cells via Enzymatic Digestion
نویسندگان
چکیده
منابع مشابه
Migratory, and not lymphoid-resident, dendritic cells maintain peripheral self-tolerance and prevent autoimmunity via induction of iTreg cells.
There is evidence that dendritic cells (DCs) induce peripheral tolerance. Nevertheless, it is not known whether immature DCs in general are able to tolerize CD4(+) T cells or if this is a prerogative of specialized subtypes. Here we show that, when autoantigen presentation is extended to all conventional mouse DCs, immature lymphoid tissue resident DCs are unable to induce autoantigen-specific ...
متن کاملCharacterization of resident and migratory dendritic cells in human lymph nodes
Dendritic cells (DCs) initiate adaptive immune responses in lymph nodes (LNs). In mice, LN DCs can be divided into resident and tissue-derived populations, the latter of which migrate from the peripheral tissues. In humans, different subsets of DCs have been identified in the blood, spleen, and skin, but less is known about populations of resident and migratory tissue-derived DCs in LNs. We hav...
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Background: Falciparum malaria is a severe health burden worldwide. Antigen presenting cells are reported to be affected by erythrocytic stage of the parasite. Malarial hemozoin (HZ), a metabolite of malaria parasite, has adjuvant properties and may play a role in the induction of immune response against the parasite. Objective: To determine the immunological impact of hemozoin on the capacity ...
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The identification of the specific DC subsets providing a critical role in presenting influenza antigens to naïve T cell precursors remains contentious and under considerable debate. Here we show that CD8(+) T lymphocyte (TCD8+) responses are severely hampered in C57BL/6 mice deficient in the transcription factor Batf3 after intranasal challenge with influenza A virus (IAV). This transcription ...
متن کاملmorphological and cytochemical characteristics of purified murine splenic dendritic cells
dendritic cells function as the main cellular population responsible for professional antigen presentation and hence for induction of primary immune responses. although they are present in virtually every tissue, nevertheless their number is usually so low that it makes their isolation for studies very difficult. in this study, we purified dendritic cells from mouse spleen by a three-step enric...
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ژورنال
عنوان ژورنال: Current Protocols in Immunology
سال: 2018
ISSN: 1934-3671,1934-368X
DOI: 10.1002/cpim.45